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Below are the instructions for this lab report. Its QUESTIONS AND ANSWER STYLE.

by | Jun 21, 2022 | Biology

 

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Below are the instructions for this lab report. Its QUESTIONS AND ANSWER STYLE. NO SOURCES IS NEEDED. I attempted to answer these questions and I’m not sure if the answers are correct, but I’m sure with your help we’ll get there. I will also provide the lab manual for a better understanding
You are going to answer Study Questions from lab handout #5 and use the data and semi-log paper provided here to find out the size (MW) of unknown proteins 1 and 2 as well as the rabbit serum protein albumen.
1)On semilog paper/graph (provided in a separate file) plot the distance migrated by each standard protein as a function of molecular weight. Determine the molecular weight of the unknown protein and the major proteins found in rabbit serum (albumin) from this calibration curve. 2)Interferon is a protein of 25000 Daltons. How far would it have migrated; had it been included in this experiment?
3)The average amino acid residue in a protein has a molecular weight of 120 Daltons. Assuming that your unknowns are average, how many amino acid residues do they contain?
Marker Proteins Molecular Weight (daltons) Distance
Serum Albumin (Dimer) 132,000 23mm
Serum Albumin (Monomer) 66,000 32mm
Ovalbumin 43,000 39mm
Lysozyme 14,400 60mm
Unknown 1 ? 26mm
Unknown 2 ? 58mm
Rabbit serum (albumin) ? 33mm

 

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